Hematology

chronic myeloid leukemia-5th edition 2023

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CML WHO 5th edition UPDATE- key points

Acclerated phase is no longer required .

Resistance to TKI ( tyrosine kinase is important prognostic factor than Acclerated phase)

criteria for blast phase in CML

>/= 20 % Myeloid Blast in blood or bone marrow.

Presence of extramedullary prolioferation of blast

Presence of increased Lymphoblast in peripheral blood or bone marrow optial cytoff forlymphoblast is yet to be established , require more studies

The significance of low-level B-lymphoblasts remain unclear and require additional studies.

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CML WHO 5th edition UPDATE- key points

Acclerated phase is no longer required .

Resistance to TKI ( tyrosine kinase is important prognostic factor than Acclerated phase)

criteria for blast phase in CML

>/= 20 % Myeloid Blast in blood or bone marrow.

Presence of extramedullary prolioferation of blast

Presence of increased Lymphoblast in peripheral blood or bone marrow optial cytoff forlymphoblast is yet to be established , require more studies

The significance of low-level B-lymphoblasts remain unclear and require additional studies.

Read more

sickling test |Sickling test procedure

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Introduction : Sickle cell disease is an inherited hemoglobin disorder  caused by a mutation in the sixth amino acid of the beta globin chain gene .  The molecular change in sickle cell  disease (SCD) is base substitution of valine for glutamate in the sixth position of beta globulin gene leading to production of abnormal hemoglobin S. HbS is … Read more

Reticulocyte count – procedure, corrected reticulocyte count

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Introduction : A reticulocyte count is a laboratory test that measures the number of immature red blood cells, known as reticulocytes, in the bloodstream. New methylene blue or brilliant cresyl blue (supravital stains) allow visualization of the reticulin network of erythrocyte  ribosome in  newly formed red blood cells Why Is Reticulocyte Count Important? Reticulocyte is … Read more

Preparation and staining of peripheral blood smear(PS)|A Step-by-Step Guide: Peripheral Blood Smear Preparation and Staining

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Introduction: Peripheral blood smear preparation and staining is a fundamental technique used in clinical laboratories to examine the cellular components of blood. It involves spreading a thin layer of blood on a glass slide, fixing the cells, and applying appropriate stains to enhance the visibility of different blood cell types. This article will guide the … Read more

Hematology cell counters(coulters)

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Hematology Coulter cell counters are laboratory instruments used to count and analyze blood cells. They are based on the principle of electrical impedance and light scattering , where cells passing through a narrow orifice generate an electrical pulse that is proportional to their size and volume. There are several types of hematology Coulter cell counters … Read more