Vitamin E is a fat soluble vitamin ,Vitamin E is best known for its antioxidant properties, which help neutralize free radicals. Free radicals are unstable molecules produced as a byproduct of normal metabolism or in response to environmental factors like pollution or UV radiation. These molecules can damage cells and contribute to aging and various chronic diseases. Vitamin E protects cell membranes, lipids, and proteins from oxidative damage by scavenging free radicals, thereby reducing oxidative stress.
when is vitamin E testing ordered or done?
patients presenting with neurological signs and symptoms such as hyporeflexia,ataxia, dementia ,muscle weakness,hemolytic anemia.
vitamin E deficiency is seen in individuals with fat malabsorption,cystic fibrosis, abetalipoproteinemia, short-bowel syndrome,Chronic cholestatic hepatobiliary disease.
sample requirement:
EDTA plasma/serum tube should be wrapped in aluminium foil . Transport temperature: 2-8 c
What precautions should be taken before vitamin E testing in patient preparation?
Patient should fast for 12 hours and abstain from alcohol for 24 hours prior to collection.
Methodology of Vitamin E Testing:
HPLC and LC-MS/MS are generally considered more accurate and specific compared to spectrophotometric or immunoassay methods.
Interpreting Vitamin E Test Results
Interpretation of vitamin E test results depends on the method used and the individual’s health status:
- Normal Range: Reference ranges for vitamin E levels can vary by age, sex, and assay method. Generally, levels below the lower limit of normal may indicate deficiency.
- Clinical Context: Test results should be interpreted alongside clinical symptoms and other laboratory tests to assess overall nutritional status and health implications.
Decreased vitamin E levels are seen in :
Premature and low birth weight infants, fat malabsorption seen in diseases like cystic fibrosis,biliary atresia, chronic cholestasis.
Increased than normal levels of vitamin E are rare and seen in excess consumption of vitamin E supplements.