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Dr.Patel Purpose To detect eosinophils in urine Materials and ReagentsHansel’s Stain (prepared as below) Preparation of Hansel’s Stain Solution (if preparing manually) 1. Eosin Y Solution (0.5%) 2. Methylene Blue Solution (0.25%) 3. Hansel Stain Working Solution Specimen Collection and Handling 🔬 Staining Procedure Microscopy and Interpretation 🟢 Normal: 0–1%🔴 Significant eosinophiluria: >1%🔴 Highly suggestive of … Read more
When collecting blood into a citrate bulb (commonly used for coagulation tests like PT, APTT, etc.), the standard anticoagulant-to-blood ratio is 1 part citrate to 9 parts blood. However, if the patient has a significantly elevated hematocrit (usually above 55%), this ratio needs to be adjusted because the plasma volume (the actual liquid portion of … Read more
H. pylori Stool Antigen Test H. pylori Antibody Test (Serology) Which one is better?
Vitamin E is a fat soluble vitamin ,Vitamin E is best known for its antioxidant properties, which help neutralize free radicals. Free radicals are unstable molecules produced as a byproduct of normal metabolism or in response to environmental factors like pollution or UV radiation. These molecules can damage cells and contribute to aging and various … Read more
Introduction Perl’s staining, also known as Prussian blue staining, is a specialized histochemical technique widely used in histopathology to detect the presence of iron in biological tissues. This method is particularly valuable in diagnosing various diseases related to iron metabolism, such as hemochromatosis and hemosiderosis. The staining highlights iron deposits, allowing pathologists to visualize and … Read more
Zinc is an essential trace mineral, meaning that the body needs it in small amounts but cannot produce it on its own. It plays a pivotal role in numerous biological processes, acting as a cofactor for over 300 enzymes involved in various functions such as metabolism, immune function, DNA synthesis, and wound healing. Additionally, zinc … Read more
What is C-peptide? C peptide originates in pancreatic beta cells as a byproduct of enzymatic cleavage of pro-insulin to insulin .C-peptide is a connecting peptide that gets released alongside insulin from the pancreas. While insulin helps usher glucose (sugar) from the bloodstream into cells for energy, C-peptide itself has no known function in the body. … Read more
A serum lipase test is most commonly ordered to diagnose acute pancreatitis. Acute pancreatitis is a sudden and severe inflammation of the pancreas, a large gland located behind the stomach. serum lipase levels are a vital for diagnosing and monitoring pancreatic disorders. Lipase in diagnosis of pancreatitis Serum amylase and lipase levels are typically elevated … Read more
MICROSCOPY The smears of squamous cell carcinoma or metastatic Squamous cell carcinoma show clusters and sheets of malignant squamous cells accompanied by mature looking squamous epithelial cells. The cells exhibit marked pleomorphism, hyperchromasia, and irregular nuclear contours. Numerous keratinized and non-keratinized squamous cells are observed. Some cells display prominent nucleoli. The nuclei show irregular chromatin … Read more