chronic myeloid leukemia-5th edition 2023

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CML WHO 5th edition UPDATE- key points

Acclerated phase is no longer required .

Resistance to TKI ( tyrosine kinase is important prognostic factor than Acclerated phase)

criteria for blast phase in CML

>/= 20 % Myeloid Blast in blood or bone marrow.

Presence of extramedullary prolioferation of blast

Presence of increased Lymphoblast in peripheral blood or bone marrow optial cytoff forlymphoblast is yet to be established , require more studies

The significance of low-level B-lymphoblasts remain unclear and require additional studies.

Read more

CML WHO 5th edition UPDATE- key points

Acclerated phase is no longer required .

Resistance to TKI ( tyrosine kinase is important prognostic factor than Acclerated phase)

criteria for blast phase in CML

>/= 20 % Myeloid Blast in blood or bone marrow.

Presence of extramedullary prolioferation of blast

Presence of increased Lymphoblast in peripheral blood or bone marrow optial cytoff forlymphoblast is yet to be established , require more studies

The significance of low-level B-lymphoblasts remain unclear and require additional studies.

Read more

Drug of abuse test in Urine

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DRUG OF ABUSE IN URINE RAPID TEST

Drug of abuse tests in urine are commonly conducted to detect the presence of specific drugs or their metabolites in an individual’s system. These tests are often used in various settings, including employment screenings, legal situations, and healthcare settings. The primary types of drug tests for urine include: Principle behind immunoassay tests involves the use … Read more

Serum Amylase test

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Serum amylase test is a blood test that measures the level of amylase in your body. Serum amylase is an easily and rapidly performed test that is commonly used to diagnose and monitor the treatment of pancreatitis or obstruction of the pancreatic duct. Amylase is an essential enzyme that plays a crucial role in the … Read more

Nephelometry- Principle,Uses in biochemistry ,limitations, difference between Nephelometry and Turbidometry

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Nephelometer for antibodies, proteins detection

Nephelometry is a laboratory technique used to measure the concentration of particles suspended in a liquid, particularly in the field of clinical chemistry and immunology. It is based on the principle of light scattering by particles in a sample, and it provides quantitative information about the amount and size distribution of the particles. In nephelometry, … Read more

BLOOD AMMONIA LEVEL TESTING

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Blood ammonia level test measures the amount of ammonia in the blood. Ammonia is a waste product produced by the body during the breakdown of proteins and is normally processed by the liver. The test is often used to evaluate the function of the liver, as the liver is responsible for converting ammonia into a … Read more

DIRECT COOMBS TEST/ANTIGLOBULIN TEST PROCEDURE

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COOMBS TEST

Described in 1945 by coombs ,mourant and race, Coombs test, also known as the antiglobulin test, is a laboratory procedure used to detect the presence of antibodies on the surface of red blood cells (RBCs). There are two main types of Coombs tests: the Direct Coombs Test (Direct Antiglobulin Test or DAT) and the Indirect … Read more

Fructosamine assay-utility

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Understanding Fructosamine: Fructosamine is generated when glucose reacts with the amino groups of proteins, primarily albumin, in a process known as the Amadori rearrangement. This reaction involves the reversible formation of a Schiff base, followed by a series of rearrangements and stabilization, resulting in the formation of fructosamine. The reaction occurs spontaneously and depends on … Read more

HS-CRP(High senstivity CRP) TEST

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Clinical significance: Hs-CRP also termed CRP cardiac is recognized as independent predictor of  increased  risk of cardiovascular disease and an adjunct to the major risk factor like smoking, diabetes, dyslipidemia by American heart association.  hs-CRP was found to be a potential mediator in the association between adiposity and Fatty Liver disease3. CRP is a protein … Read more

AFP test -tumor marker

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AFP test assay is intended for the use as: ▪ An aid in the diagnosis of hepatocellular carcinoma (HCC). ▪ An aid in the management of patients with non‑seminomatous germ cell tumors Clinical significance: AFP ( alpha-fetoprotein) is a glycoprotein that is normally produced during gestation by fetal liver yolksac, and the fetal gastrointestinal tract . … Read more

CA-125 TEST INFO

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Clinical significance: CA-125 is a large glycoprotein which promotes cancer cell proliferation and inhibits anticancer immune responses. CA-125 is found in high percentage of ovarian tumors of epithelial origin(non mucinous) and can be detected in serum. This test can be used to: Clinical uses: 1. Aids in detection of residual or recurrent ovarian carcinoma in … Read more