Order of blood Draw-CLSI guidelines

The Order of Blood Draw in Phlebotomy

Order of blood draw in multiple tube collection CLSI guidelines

The Clinical and Laboratory Standards Institute (CLSI) has a guideline on the order of blood draw. The guideline is called GP41: Collection of Diagnostic Venous Blood Specimens. The guideline recommends the following order of draw:

Phlebotomy is the process of drawing blood from a vein. It is a common medical procedure that is used to collect blood for a variety of tests. When drawing blood, it is important to follow the correct order of draw to avoid cross-contamination of the samples.

The order of blood draw is as follows:

  1. Blood Culture Tubes or Vials-
  2. Coagulation Tubes (Blue-Top Tubes)Sodium citrate tubes. 
  3. Serum Tubes without Clot Activator or Gel (Red-Top tubes)
  4. Serum Tubes with Clot Activator or Gel (Gold or Tiger Top Tubes)
  5. Heparin Tubes (Green-Top Tubes)
  6. EDTA Tubes (Lavender-Top Tubes)
  7. Fluoride Tubes (Gray-Top Tubes)
Color of the capAnticoagulantorder

Transport Media FIRST
3.2 % Sodium
None (Tubes with
gel separator &
clot activator) red or yellow top
Sodium fluoride &
Potassium oxalate

For more information on basics of phlebotomy read on https://journals.lww.com/nursingmadeincrediblyeasy/fulltext/2016/07000/phlebotomy_basics.8.aspx

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